A benchtop single molecule localization microscopy (SMLM) system integrated with a dedicated workflow for the detection and analysis of extracellualr vesicles (EVs) including; • Automated EV-specific acquisition software; • Single-EV super-resolution imaging and quantitative biomarker analysis within a unified workflow; • Automated EV-focused reporting and analysis; and • A complete solution encompassing reagents, acquisition, analysis, and reporting designed specifically for EV characterization.