TU Delft requires a 300 kV cryogenic transmission electron microscope combining capabilities for established single-particle analysis and cryo-electron tomography with advanced scanning transmission electron microscopy (STEM), including low-dose 4D-STEM techniques. These complementary methods will enable the investigation of biological structures across different length scales while addressing limitations imposed by specimen thickness and radiation sensitivity. The microscope will operate as a shared research infrastructure and support both routine, automated data acquisition by multiple users across different experience levels and the development of new imaging methods. The acquisition will replace the existing microscope and must provide a fully integrated platform for current and future research activities. The following minimum (KO) requirements have been established to ensure that the instrument meets these scientific and operational needs. See attached document for more info. 1